Journal: Advanced healthcare materials

Article Title: Designing Inherently Photodegradable Cell-Adhesive Hydrogels for 3D Cell Culture.

doi: 10.1002/adhm.202100632

Figure Lengend Snippet: Figure 2. A) Live/dead staining of normal human dermal fibroblasts (NHDF) with calcein-AM (green, live cells) and propidium iodide (red, dead cells) embedded in GelMA-free PEGMA/PEGDMA (Gel-N0) and GelMA-containing (Gel-N10) hydrogels on day 1, 7, 14 postencapsulation, followed by imaging with confocal microscopy (Leica TCS SPE, scale bar: 200 µm). B-i) NHDF embedded in Gel-N10 hydrogels on day 1 postencapsulation. Comparison of HepG2 cells encapsulated in (ii) Gel-N10 and (iii) Gel-N0 hydrogels (scale bar: 100 µm). C) Proliferation behavior of encapsulated HepG2 cells in Gel-N10 and Gel-N0 hydrogels over a period of 7 d assessed by PrestoBlue assay (n = 3). D) Toxicity evaluation of Gel-N10 photodegradation products by MTT proliferation assay with HeLa cells after 72 h exposure (n = 3). The concentration of nondiluted degradation products was 53 mg mL−1. Data are presented as mean ± SD and statistically evaluated by (C) Student’s t-test and (D) one-way ANOVA. *, **, *** represent p < 0.05, 0.01, and 0.001, respectively.

Article Snippet: Normal human dermal fibroblasts (NHDF), human liver cancer cells (HepG2), and HeLa cells were purchased from PromoCell GmbH (Heidelberg, Germany).

Techniques: Staining, Imaging, Confocal Microscopy, Comparison, Prestoblue Assay, Proliferation Assay, Concentration Assay

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: The Induction of Apoptosis in A375 Malignant Melanoma Cells by Sutherlandia frutescens

doi: 10.1155/2016/4921067

Figure Lengend Snippet: The effect of S. frutescens extract on the viability of (a) A375 melanoma, (b) Colo-800 melanoma, (c) HDF α fibroblast, and (d) Hek 293 cells after 24, 48, and 72 h of treatment. The viability was assessed using the alamarBlue assay and expressed as percentage relative to the control-treated cells (0 mg/mL). Error bars represent the SEM ( n ≥ 3) and ∗ indicates a significant difference from 0 mg/mL ( P < 0.05).

Article Snippet: The human primary dermal fibroblast cell line (HDF α ) was purchased from ScienCell, CA, USA.

Techniques: Alamar Blue Assay, Control

Journal: Cancer Science

Article Title: Caffeine yields aneuploidy through asymmetrical cell division caused by misalignment of chromosomes

doi: 10.1111/j.1349-7006.2008.00862.x

Figure Lengend Snippet: Transient mitotic arrest induced by 10 mmol/L caffeine. (a) HeLa cells were synchronized at G2 phase (0 h) and then treated with 0, 2, and 10 mmol/L caffeine simultaneously with or without 2 µg/mL nocodazole for 4 h. The percentages of G1‐phase fractions, G2‐phase fractions, and phosphohistone H3 (Ser10)‐positive M‐phase fractions are indicated. (b) HeLa cells were synchronized at G2 phase (0 h) and then treated with or without 10 mmol/L caffeine for 12 h. The percentages of sub‐G1, G1 and early S, late S and G2, and phosphohistone H3 (Ser10)‐positive M‐phase fractions are indicated. (c) Stills of time‐lapse live‐cell microscopy of HeLa cells treated with or without 10 mmol/L caffeine. (d) The boxplots for the duration of mitosis of HeLa (left), primary human fibroblast (middle), and U2OS (right) cells in the presence or absence of 10 mmol/L caffeine. n = 30 for each.

Article Snippet: Primary normal human dermal fibroblasts were obtained from Cambrex Bio Science and grown in FGM‐2 SingleQuots medium supplemented with 2% FBS, human fibroblast growth factor‐basic, insulin, gentamicin, and amphotericin‐B.

Techniques: Microscopy